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验证数据展示

  • WB analysis using 22033-1-AP

    WB analysis using 22033-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22033-1-AP (SARA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 22033-1-AP

    IHC staining of human kidney using 22033-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 22033-1-AP (SARA antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 22033-1-AP

    IHC staining of human kidney using 22033-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 22033-1-AP (SARA antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of mouse brain using 22033-1-AP

    IP experiment of mouse brain using 22033-1-AP

    IP result of anti-SARA (IP:22033-1-AP, 5ug; Detection:22033-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

  • IF Staining of mouse brain using 22033-1-AP

    IF Staining of mouse brain using 22033-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SARA antibody (22033-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse kidney using 22033-1-AP

    IF Staining of mouse kidney using 22033-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SARA antibody (22033-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

SARA Polyclonal antibody

SARA Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 22033-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA and More (1)

别名

hSARA, Madh interacting protein, MADHIP, Novel serine protease, NSP, Receptor activation anchor, SARA, SMADIP, ZFYVE9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, MCF-7 cells, human brain tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue, mouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22033-1-AP targets SARA in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SARA fusion protein Ag16929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, FYVE domain containing 9
别名 hSARA, Madh interacting protein, MADHIP, Novel serine protease, NSP, Receptor activation anchor, SARA, SMADIP, ZFYVE9
计算分子量 1425 aa, 156 kDa
观测分子量 156 kDa
GenBank蛋白编号BC032680
基因名称 SARA
Gene ID (NCBI) 9372
RRIDAB_11182938
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95405
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZFYVE9, also known as SARA (Smad anchor for receptor activation), is a double zinc finger (FYVE domain) protein that interacts directly with SMAD2 and SMAD3, and is involved in Alzheimer's disease. SARA functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. SARA plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. SARA is possibly associated with TGF-beta receptor internalization.

实验方案

Product Specific Protocols
WB protocol for SARA antibody 22033-1-APDownload protocol
IHC protocol for SARA antibody 22033-1-APDownload protocol
IF protocol for SARA antibody 22033-1-APDownload protocol
IP protocol for SARA antibody 22033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Structure

Structural Basis for Specific Interaction of TGFβ Signaling Regulators SARA/Endofin with HD-PTP.

Authors - Deepankar Gahloth
  • KD Validated
View Article
mouseWB,IF

J Bone Miner Res

Dysfunction of Caveolae-mediated Endocytic TβRI Degradation Results in Hypersensitivity of TGF-β/Smad Signaling in Osteogenesis Imperfecta

Authors - Changgui Shi
View Article
humanWB,IF

Sci Adv

Neuropilin 1 is an entry receptor for KSHV infection of mesenchymal stem cell through TGFBR1/2-mediated macropinocytosis

Authors - Zheng-Zhou Lu
  • KO Validated
View Article