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ZG16 Monoclonal antibody, PBS Only

ZG16 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A7B9

Cat no : 67389-1-PBS

Print datasheet

Synonyms

FLJ43571, FLJ92276, hZG16, JCLN, JCLN1, Secretory lectin ZG16, ZG16, ZG16A, Zymogen granule protein 16

验证数据展示

  • WB analysis of mouse colon using 67389-1-Ig (same clone as 67389-1-PBS)

    WB analysis of mouse colon using 67389-1-Ig (same clone as 67389-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 67389-1-Ig (ZG16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

    IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

    IHC staining of mouse pancreas using 67389-1-Ig (same clone as 67389-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67389-1-Ig (ZG16 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

    IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

    IF Staining of mouse small intestine using 67389-1-Ig (same clone as 67389-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ZG16 antibody (67389-1-Ig, Clone: 1A7B9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ASCT2 antibody (20350-1-AP, green), CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 67389-1-PBS in a different storage buffer formulation.

产品信息

67389-1-PBS targets ZG16 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, mouse, pig, rat
Immunogen ZG16 fusion protein Ag11332 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zymogen granule protein 16 homolog (rat)
Synonyms FLJ43571, FLJ92276, hZG16, JCLN, JCLN1, Secretory lectin ZG16, ZG16, ZG16A, Zymogen granule protein 16
Calculated Molecular Weight 167 aa, 18 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC029149
Gene Symbol ZG16
Gene ID (NCBI) 653808
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60844
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)