验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse ovary tissue, mouse lung tissue, rat brain tissue, Jurkat cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ZHX2 Ready-To-Use IHC Kit. ZHX2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20136-1-AP targets ZHX2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | ZHX2 fusion protein Ag14088 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc fingers and homeoboxes 2 |
| 别名 | Regulator of AFP, RAF, KIAA0854, Alpha-fetoprotein regulator 1, AFR1 |
| 计算分子量 | 837 aa, 92 kDa |
| 观测分子量 | 92-100 kDa |
| GenBank蛋白编号 | BC042145 |
| 基因名称 | ZHX2 |
| Gene ID (NCBI) | 22882 |
| RRID | AB_10666438 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6X8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| IHC protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| IF protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| IP protocol for ZHX2 antibody 20136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Cancer HBV suppresses ZHX2 expression to promote proliferation of HCC through miR-155 activation. | ||
J Pathol ZHX2 inhibits SREBP1c-mediated de novo lipogenesis in hepatocellular carcinoma via miR-24-3p. | ||
J Immunol Zhx2 Accelerates Sepsis by Promoting Macrophage Glycolysis via Pfkfb3.
| ||
Cancer Cell Int ZHX2 inhibits thyroid cancer metastasis through transcriptional inhibition of S100A14.
| ||
Biochem Biophys Res Commun Proliferation and osteo/odontogenic differentiation of stem cells from apical papilla regulated by Zinc fingers and homeoboxes 2: An in vitro study.
| ||
FEBS Open Bio Upregulation of ZHX2 predicts poor prognosis and is correlated with immune infiltration in gastric cancer. |
