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ZIC3 Polyclonal antibody

ZIC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21717-1-AP

Print datasheet

Synonyms

ZNF203, Zinc finger protein ZIC 3

验证数据展示

  • WB analysis of Neuro-2a using 21717-1-AP

    WB analysis of Neuro-2a using 21717-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21717-1-AP (ZIC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 21717-1-AP

    IHC staining of mouse brain using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZIC3 antibody included
  • IHC staining of rat brain using 21717-1-AP

    IHC staining of rat brain using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZIC3 antibody included
  • IHC staining of rat cerebellum using 21717-1-AP

    IHC staining of rat cerebellum using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZIC3 antibody included
  • IHC staining of human urothelial carcinoma using 21717-1-AP

    IHC staining of human urothelial carcinoma using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZIC3 antibody included
  • IHC staining of mouse cerebellum using 21717-1-AP

    IHC staining of mouse cerebellum using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZIC3 antibody included
  • IHC staining of human thyroid cancer using 21717-1-AP

    IHC staining of human thyroid cancer using 21717-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21717-1-AP (ZIC3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZIC3 antibody included

经过测试的应用

Positive WB detected inNeuro-2a cells, mouse cerebellum tissue, SH-SY5Y cells, Y79 cells
Positive IHC detected inhuman urothelial carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ZIC3 Ready-To-Use IHC Kit. ZIC3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21717-1-AP targets ZIC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ZIC3 fusion protein Ag16354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Zic family member 3 (odd-paired homolog, Drosophila)
Synonyms ZNF203, Zinc finger protein ZIC 3
Calculated Molecular Weight 467 aa, 51 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC113395
Gene Symbol ZIC3
Gene ID (NCBI) 7547
RRIDAB_2878910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60481
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZIC3 antibody 21717-1-APDownload protocol
IHC protocol for ZIC3 antibody 21717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols