Phospho-ZIPK (Ser311) Polyclonal antibody
Phospho-ZIPK (Ser311) Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DAPK3 Ready-To-Use IHC Kit. DAPK3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
51021-1-AP targets Phospho-ZIPK (Ser311) in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | death-associated protein kinase 3 |
| Synonyms | DAP kinase 3, DAP like kinase, DAPK3, Dlk, Phospho-ZIPK (Ser311), ZIP, ZIP kinase, ZIPK |
| Calculated Molecular Weight | 52.5 kDa |
| Observed Molecular Weight | 52.5 kDa |
| GenBank Accession Number | BC126430 |
| Gene Symbol | DAPK3 |
| Gene ID (NCBI) | 1613 |
| RRID | AB_614997 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43293 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DAPK3(Death-associated protein kinase 3) is also named ZIPK(Zipper-interacting protein kinase), and Dlk (DAP-like kinase). It is a member of the death-associated protein kinase family associated with apoptosis in nonmuscle cells where it phosphorylates myosin regulatory light chain (RLC) to promote membrane blebbing. DAPK3 mRNA and protein are abundant in heart tissue and isolated ventricular neonatal rat cardiac myocytes(PMID:20038585). It has 2 isoforms produced by alternative splicing, named as ZIPK-L and ZIPK-S. This antibody is specific to ZIPK-Phospho 311Ser.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-ZIPK (Ser311) antibody 51021-1-AP | Download protocol |
| IHC protocol for Phospho-ZIPK (Ser311) antibody 51021-1-AP | Download protocol |
| IP protocol for Phospho-ZIPK (Ser311) antibody 51021-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Signal Phosphorylation-dependent control of ZIPK nuclear import is species specific. |