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验证数据展示

  • WB analysis using 25214-1-AP

    WB analysis using 25214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25214-1-AP (ZKSCAN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 25214-1-AP

    WB analysis of HEK-293 using 25214-1-AP

    WB result of ZKSCAN1 antibody (25214-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZKSCAN1 transfected HEK-293 cells.

  • IF Staining of A431 using 25214-1-AP

    IF Staining of A431 using 25214-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZKSCAN1 antibody (25214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

ZKSCAN1 Polyclonal antibody

ZKSCAN1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25214-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

9130423L19Rik, KOX18, PHZ 37, Zinc finger protein 139, Zinc finger protein 36, Zinc finger protein KOX18, ZKSCAN1, ZNF139, ZNF36

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25214-1-AP targets ZKSCAN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ZKSCAN1 fusion protein Ag19721 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger with KRAB and SCAN domains 1
Synonyms 9130423L19Rik, KOX18, PHZ 37, Zinc finger protein 139, Zinc finger protein 36, Zinc finger protein KOX18, ZKSCAN1, ZNF139, ZNF36
Calculated Molecular Weight 563 aa, 64 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC022378
Gene Symbol ZKSCAN1
Gene ID (NCBI) 7586
RRIDAB_2879963
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17029
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZKSCAN1(Zinc finger protein with KRAB and SCAN domains 1), also named as ZNF139, is a member in transcription factor zinc finger protein family(PMID: 24515389). Zinc finger protein 139 (ZNF139) gene is proved play an important role in gastric cancer, overexpression of ZNF139 correlated with tumor differentiation, invasion depth, clinical stage, lymphatic metastasis, and blood vessel invasion(PMID: 25436386, PMID: 25561801). The molecular weight of ZKSCAN1 is 64 kDa.

实验方案

Product Specific Protocols
WB protocol for ZKSCAN1 antibody 25214-1-APDownload protocol
IF protocol for ZKSCAN1 antibody 25214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Lett

Oncogenic functions and therapeutic potentials of targeted inhibition of SMARCAL1 in small cell lung cancer

Authors - Bei-Bei Sun
View Article