验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ZMYND8 Ready-To-Use IHC Kit. ZMYND8 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11633-1-AP targets ZMYND8 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ZMYND8 fusion protein Ag2207 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, MYND-type containing 8 |
| 别名 | CTCL associated antigen se14 3, KIAA1125, PRKCBP1, PRO2893, RACK7, ZMYND8 |
| 计算分子量 | 1186 aa, 132 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC030721 |
| 基因名称 | ZMYND8 |
| Gene ID (NCBI) | 23613 |
| RRID | AB_2241834 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULU4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins including REST corepressors (RCOR). ZMYND8 bind to ROCR2 and can function as transcriptional repressors. It is co-expressed with RCOR2 in the nervous system and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974]
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZMYND8 antibody 11633-1-AP | Download protocol |
| IHC protocol for ZMYND8 antibody 11633-1-AP | Download protocol |
| IP protocol for ZMYND8 antibody 11633-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Mol Cell The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability.
| ||
Mol Cancer LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.
| ||
Cancer Res KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity | ||
Sci Adv The endogenous retrovirus-derived long noncoding RNA TROJAN promotes triple-negative breast cancer progression via ZMYND8 degradation. |
