• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ZNF101 Polyclonal antibody

ZNF101 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25599-1-AP

Print datasheet

Synonyms

HZF12

验证数据展示

  • WB analysis of HepG2 using 25599-1-AP

    WB analysis of HepG2 using 25599-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25599-1-AP (ZNF101 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25599-1-AP

    IF Staining of HeLa using 25599-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF101 antibody (25599-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of human stomach cancer using 25599-1-AP

    IHC staining of human stomach cancer using 25599-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25599-1-AP (ZNF101 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZNF101 antibody included

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ZNF101 Ready-To-Use IHC Kit. ZNF101 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25599-1-AP targets ZNF101 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ZNF101 fusion protein Ag22390 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 101
Synonyms HZF12
Calculated Molecular Weight 436 aa, 50 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC119680
Gene Symbol ZNF101
Gene ID (NCBI) 94039
RRIDAB_2880152
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF101 antibody 25599-1-APDownload protocol
IHC protocol for ZNF101 antibody 25599-1-APDownload protocol
IF protocol for ZNF101 antibody 25599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols