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ZNF124 Polyclonal antibody

ZNF124 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25216-1-AP

Print datasheet

Synonyms

HZF 16, HZF16, zinc finger protein 124, Zinc finger protein HZF 16, ZNF124

验证数据展示

  • WB analysis of SH-SY5Y using 25216-1-AP

    WB analysis of SH-SY5Y using 25216-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25216-1-AP (ZNF124 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 25216-1-AP

    IP experiment of SH-SY5Y using 25216-1-AP

    IP result of anti-ZNF124 (IP:25216-1-AP, 4ug; Detection:25216-1-AP 1:2000) with SH-SY5Y cells lysate 1600ug.

  • IHC staining of human gliomas using 25216-1-AP

    IHC staining of human gliomas using 25216-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25216-1-AP (ZNF124 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 25216-1-AP

    IF Staining of HepG2 using 25216-1-AP

    Immunofluorescent analysis of HepG2 cells using 25216-1-AP (ZNF124 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSH-SY5Y cells, U-937 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25216-1-AP is ZNF124 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen ZNF124 fusion protein Ag17165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 124
Synonyms HZF 16, HZF16, zinc finger protein 124, Zinc finger protein HZF 16, ZNF124
Calculated Molecular Weight 351 aa, 40 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC099661
Gene Symbol ZNF124
Gene ID (NCBI) 7678
RRIDAB_2879965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15973
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF124 antibody 25216-1-APDownload protocol
IHC protocol for ZNF124 antibody 25216-1-APDownload protocol
IF protocol for ZNF124 antibody 25216-1-APDownload protocol
IP protocol for ZNF124 antibody 25216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols