验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16618-1-AP targets ZNF143 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ZNF143 fusion protein Ag9914 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 143 |
| 别名 | hStaf, pHZ 1, SBF, SPH binding factor, STAF, zinc finger protein 143, ZNF143 |
| 计算分子量 | 626 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC020219 |
| 基因名称 | ZNF143 |
| Gene ID (NCBI) | 7702 |
| RRID | AB_2218324 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P52747 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zinc finger protein143(ZNF143) contains a separete acidic activation and 7 zinc finger domains. It's a transcription factor that required for activation of the majority of vertebrate snRNA and snRNA-type genes, which transcribed by RNA pol II and pol III. Also, it binds to the SPH motif of small nuclear RNA (snRNA) gene promoters and participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF143 antibody 16618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo. | ||
Mol Cell Architectural Proteins and Pluripotency Factors Cooperate to Orchestrate the Transcriptional Response of hESCs to Temperature Stress. | ||
Nat Commun Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy. | ||
Proc Natl Acad Sci U S A Cooperative transcription factor associations discovered using regulatory variation. | ||
Cancer Res ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.
| ||
Cell Rep Host Cell Factor-1 Recruitment to E2F-Bound and Cell-Cycle-Control Genes Is Mediated by THAP11 and ZNF143.
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