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ZNF146 Polyclonal antibody

ZNF146 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20385-1-AP

Print datasheet

Synonyms

Only zinc finger protein, OZF, zinc finger protein 146, Zinc finger protein OZF, ZNF146

验证数据展示

  • WB analysis of mouse kidney using 20385-1-AP

    WB analysis of mouse kidney using 20385-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20385-1-AP (ZNF146 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 20385-1-AP

    IHC staining of human heart using 20385-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20385-1-AP (ZNF146 antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inmouse kidney tissue, mouse lung tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20385-1-AP is ZNF146 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ZNF146 fusion protein Ag14223 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 146
Synonyms Only zinc finger protein, OZF, zinc finger protein 146, Zinc finger protein OZF, ZNF146
Calculated Molecular Weight 292 aa, 33 kDa
Observed Molecular Weight 20-25 kDa, 33-35 kDa
GenBank Accession NumberBC005154
Gene Symbol ZNF146
Gene ID (NCBI) 7705
RRIDAB_10694154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15072
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF146 antibody 20385-1-APDownload protocol
IHC protocol for ZNF146 antibody 20385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanChIP

Reproduction

ZNF146 regulates cell cycle progression via TFDP1 and DEPDC1B in ovarian cancer cells

Authors - Ruixue Zhao
View Article
human,mouseWB,IHC

J Gastrointest Oncol

Hsa_circ_NOTCH3 regulates ZNF146 through sponge adsorption of miR-875-5p to promote tumorigenesis of hepatocellular carcinoma.

Authors - Lei Bao
View Article