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验证数据展示

  • WB analysis of BxPC-3 using 18157-1-AP

    WB analysis of BxPC-3 using 18157-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 18157-1-AP (ZNF174 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 18157-1-AP

    IHC staining of human testis using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 18157-1-AP

    IHC staining of human testis using 18157-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18157-1-AP (ZNF174 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 18157-1-AP

    IF Staining of HeLa using 18157-1-AP

    Immunofluorescent analysis of Hela cells, using ZNF174 antibody 18157-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis using 18157-1-AP

    WB analysis using 18157-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18157-1-AP (ZNF174 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

ZNF174 Polyclonal antibody

ZNF174 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18157-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

AW 1, zinc finger protein 174, ZNF174, ZSCAN8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman testis tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18157-1-AP targets ZNF174 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZNF174 fusion protein Ag12963 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 174
别名 AW 1, zinc finger protein 174, ZNF174, ZSCAN8
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC000876
基因名称 ZNF174
Gene ID (NCBI) 7727
RRIDAB_2218459
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15697
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF174 antibody 18157-1-APDownload protocol
IHC protocol for ZNF174 antibody 18157-1-APDownload protocol
IF protocol for ZNF174 antibody 18157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols