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ZNF202 Polyclonal antibody

ZNF202 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26216-1-AP

Print datasheet

Synonyms

ZKSCAN10, Zinc finger protein 202

验证数据展示

  • WB analysis using 26216-1-AP

    WB analysis using 26216-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26216-1-AP (ZNF202 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 26216-1-AP

    WB analysis using 26216-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26216-1-AP (ZNF202 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse spermatophore using 26216-1-AP

    WB analysis of mouse spermatophore using 26216-1-AP

    mouse spermatophore tissue were subjected to SDS PAGE followed by western blot with 26216-1-AP (ZNF202 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 26216-1-AP

    IHC staining of mouse testis using 26216-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26216-1-AP (ZNF202 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, human testis tissue, mouse spermatophore tissue, HeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26216-1-AP targets ZNF202 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ZNF202 fusion protein Ag23859 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 202
Synonyms ZKSCAN10, Zinc finger protein 202
Observed Molecular Weight49 kDa
GenBank Accession NumberBC013382
Gene Symbol ZNF202
Gene ID (NCBI) 7753
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95125
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF202 gene product is a transcriptional repressor that binds to elements found predominantly in genes that participate in lipid metabolism. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), and several genes involved in processes related to energy metabolism and vascular disease (PMID: 10748193). ZNF202 is expressed in two common splice variants. The α protein consists of 424 amino acids, while the β-protein consists of 648 amino acids (PMID: 11279031).

实验方案

Product Specific Protocols
WB protocol for ZNF202 antibody 26216-1-APDownload protocol
IHC protocol for ZNF202 antibody 26216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols