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ZNF217 Monoclonal antibody

ZNF217 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F11B6

Cat no : 67730-1-Ig

Print datasheet

Synonyms

ZABC1, zinc finger protein 217, ZNF217

验证数据展示

  • WB analysis using 67730-1-Ig

    WB analysis using 67730-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67730-1-Ig (ZNF217 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67730-1-Ig

    WB analysis of HEK-293 using 67730-1-Ig

    WB result of ZNF217 antibody (67730-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF217 transfected HEK-293 cells.

  • IHC staining of mouse skin using 67730-1-Ig

    IHC staining of mouse skin using 67730-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67730-1-Ig (ZNF217 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skin using 67730-1-Ig

    IHC staining of mouse skin using 67730-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 67730-1-Ig (ZNF217 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, 4T1 cells, HEK-293 cells, HepG2 cells, PC-3 cells, K-562 cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67730-1-Ig targets ZNF217 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen ZNF217 fusion protein Ag19468 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zinc finger protein 217
Synonyms ZABC1, zinc finger protein 217, ZNF217
Calculated Molecular Weight 1048 aa, 115 kDa
Observed Molecular Weight110-130 kDa
GenBank Accession NumberBC113427
Gene Symbol ZNF217
Gene ID (NCBI) 7764
RRIDAB_2882916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75362
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF217 antibody 67730-1-IgDownload protocol
IHC protocol for ZNF217 antibody 67730-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols