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ZNF23 Polyclonal antibody

ZNF23 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21322-1-AP

Print datasheet

Synonyms

Zinc finger protein KOX16, Zinc finger protein 612, Zinc finger protein 359, Zinc finger protein 23, Zfp612

验证数据展示

  • WB analysis of HepG2 using 21322-1-AP

    WB analysis of HepG2 using 21322-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21322-1-AP

    IF Staining of HepG2 using 21322-1-AP

    Immunofluorescent analysis of HepG2 cells, using ZNF23 antibody 21322-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse brain using 21322-1-AP

    IP experiment of mouse brain using 21322-1-AP

    IP result of anti-ZNF23 (IP:21322-1-AP, 4ug; Detection:21322-1-AP 1:800) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inHepG2 cells, A2780 cells, HeLa cells, mouse brain tissue, mouse lung tissue, mouse spleen tissue, SKOV-3 cells
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21322-1-AP targets ZNF23 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ZNF23 fusion protein Ag14755 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 23 (KOX 16)
Synonyms Zinc finger protein KOX16, Zinc finger protein 612, Zinc finger protein 359, Zinc finger protein 23, Zfp612
Calculated Molecular Weight 643 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC007256
Gene Symbol ZNF23
Gene ID (NCBI) 7571
RRIDAB_2878839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17027
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF23 antibody 21322-1-APDownload protocol
IF protocol for ZNF23 antibody 21322-1-APDownload protocol
IP protocol for ZNF23 antibody 21322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cell Physiol Biochem

ZNF23 Suppresses Cutaneous Melanoma Cell Malignancy via Mitochondria-Dependent Pathway.

Authors - Xin Zhang
View Article