验证数据展示
经过测试的应用
| Positive WB detected in | mouse cerebellum tissue, mouse heart tissue, mouse brain tissue, mouse kidney tissue, rat heart tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12714-1-AP targets ZNF238/ZBTB18 in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, chIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ZNF238/ZBTB18 fusion protein Ag3406 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 238 |
| 别名 | 58 kDa repressor protein, C2H2 171, RP58, TAZ 1, TAZ1, Transcriptional repressor RP58, ZBTB18, ZFP238, zinc finger protein 238, Zinc finger protein C2H2 171, ZNF238 |
| 计算分子量 | 531 aa, 59 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC036677 |
| 基因名称 | ZNF238/ZBTB18 |
| Gene ID (NCBI) | 10472 |
| RRID | AB_2218388 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99592 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNF238 is a member of the BTB/POZ-ZF protein family, which involve in development and cancer formation, for example BCL-6, PLZF, and HIC-1. It's a transcriptional repressor involve in myogenesis and brain development. By directly repressing the expression of two skeletal myogenesis inhibitors, ID2 and ID3, ZNF238 plays a key role in myogenesis. It can control cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. Besides, ZNF238 involves in the organization of nuclear chromosomes, for its specific binding to the consensus DNA sequence that contains the E box core, and recruiting chromatin remodeling multi-protein complex. ZNF238 proteins has apparent molecular masses of 60 and 48 kD. Specific binding is found for a 60-kDa band which corresponds to the full length of RP58 protein. In addition, a 48-kDa band, thought to be the truncated form 2 is detected (PMID: 9756912).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF238/ZBTB18 antibody 12714-1-AP | Download protocol |
| IHC protocol for ZNF238/ZBTB18 antibody 12714-1-AP | Download protocol |
| IP protocol for ZNF238/ZBTB18 antibody 12714-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
PLoS Biol Olig2 and Hes regulatory dynamics during motor neuron differentiation revealed by single cell transcriptomics. | ||
Cereb Cortex The Zinc Finger Transcription Factor RP58 Negatively Regulates Rnd2 for the Control of Neuronal Migration During Cerebral Cortical Development. | ||
Commun Biol ZBTB18 regulates cytokine expression and affects microglia/macrophage recruitment and commitment in glioblastoma | ||
iScience Calpain-mediated cleavage generates a ZBTB18 N-terminal product that regulates HIF1A signaling and glioblastoma metabolism. | ||
Neural Dev Rp58 and p27(kip1) coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex. |
