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验证数据展示

  • WB analysis of HepG2 using 25152-1-AP

    WB analysis of HepG2 using 25152-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25152-1-AP (ZNF253 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ZNF253 Polyclonal antibody

ZNF253 Polyclonal Antibody for WB, ELISA
Cat No. 25152-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

BMZF 1, BMZF1, Bone marrow zinc finger 1, zinc finger protein 253, Zinc finger protein 411, ZNF253, ZNF411

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, SMMC-7721 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25152-1-AP targets ZNF253 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ZNF253 fusion protein Ag18570 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 253
别名 BMZF 1, BMZF1, Bone marrow zinc finger 1, zinc finger protein 253, Zinc finger protein 411, ZNF253, ZNF411
计算分子量 499 aa, 58 kDa
观测分子量 58-65 kDa
GenBank蛋白编号BC125063
基因名称 ZNF253
Gene ID (NCBI) 56242
RRIDAB_2879927
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75346
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF253, also named as Zinc finger protein 411 or BMZF1, is a 499 amino acid protein, which contains one KRAB domain and eleven C2H2-type zinc fingers. ZNF253 belongs to the krueppel C2H2-type zinc-finger protein family and is expressed in bone marrow and in monocytic and immature erythroid cell lines. ZNF253 may function as a transcription factor.

实验方案

Product Specific Protocols
WB protocol for ZNF253 antibody 25152-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols