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ZNF263 Polyclonal antibody

ZNF263 Polyclonal Antibody for IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21326-1-AP

Print datasheet

Synonyms

ZKSCAN12, Zinc finger protein FPM315, Zinc finger protein 263, ZFP263, FPM315

验证数据展示

  • IP experiment of HepG2 using 21326-1-AP

    IP experiment of HepG2 using 21326-1-AP

    IP result of anti-ZNF263 (IP:21326-1-AP, 4ug; Detection:21326-1-AP 1:300) with HepG2 cells lysate 4000ug.

  • IF Staining of U2OS using 21326-1-AP

    IF Staining of U2OS using 21326-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ZNF263 antibody (21326-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive IP detected inHepG2 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

ChIPSee 1 publications below

产品信息

21326-1-AP targets ZNF263 in IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IP, ELISA Application Description
Cited ApplicationsChIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen ZNF263 fusion protein Ag15158 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 263
Synonyms ZKSCAN12, Zinc finger protein FPM315, Zinc finger protein 263, ZFP263, FPM315
Calculated Molecular Weight 683 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC008805
Gene Symbol ZNF263
Gene ID (NCBI) 10127
RRIDAB_2878841
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14978
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF263 is a transcriptional repressor and activator that contains an N-terminal scanning domain and nine C2H2 zinc finger domains at the C-terminus, as well as a kruppel association box (KRAB) domain (PMID: 10567577). ZNF263 has been reported to be involved in early human embryonic development, cardiac regeneration, and adipogenesis (PMID: 37065635).

实验方案

Product Specific Protocols
IF protocol for ZNF263 antibody 21326-1-APDownload protocol
IP protocol for ZNF263 antibody 21326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanChIP

Front Oncol

Low Level of PALMD Contributes to the Metastasis of Uveal Melanoma.

Authors - Min-Yun Cai
View Article