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验证数据展示

  • WB analysis of PC-3 using 25094-1-AP

    WB analysis of PC-3 using 25094-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25094-1-AP (ZNF277 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25094-1-AP

    IF Staining of HepG2 using 25094-1-AP

    Immunofluorescent analysis of HepG2 cells using 25094-1-AP (ZNF277 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

ZNF277 Polyclonal antibody

ZNF277 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25094-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

NRIF4, zinc finger protein 277, ZNF277, ZNF277P

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25094-1-AP targets ZNF277 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZNF277 fusion protein Ag18979 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 277
别名 NRIF4, zinc finger protein 277, ZNF277, ZNF277P
计算分子量 450 aa, 53 kDa
观测分子量 53 kDa
GenBank蛋白编号BC020626
基因名称 ZNF277
Gene ID (NCBI) 11179
RRIDAB_2879893
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRM2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF277 antibody 25094-1-APDownload protocol
IF protocol for ZNF277 antibody 25094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols