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ZNF277 Polyclonal antibody

ZNF277 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25094-1-AP

Print datasheet

Synonyms

NRIF4, zinc finger protein 277, ZNF277, ZNF277P

验证数据展示

  • WB analysis of PC-3 using 25094-1-AP

    WB analysis of PC-3 using 25094-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25094-1-AP (ZNF277 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25094-1-AP

    IF Staining of HepG2 using 25094-1-AP

    Immunofluorescent analysis of HepG2 cells using 25094-1-AP (ZNF277 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inPC-3 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25094-1-AP is ZNF277 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ZNF277 fusion protein Ag18979 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 277
Synonyms NRIF4, zinc finger protein 277, ZNF277, ZNF277P
Calculated Molecular Weight 450 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC020626
Gene Symbol ZNF277
Gene ID (NCBI) 11179
RRIDAB_2879893
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRM2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF277 antibody 25094-1-APDownload protocol
IF protocol for ZNF277 antibody 25094-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols