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验证数据展示

  • WB analysis of THP-1 using 84121-5-RR

    WB analysis of THP-1 using 84121-5-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 84121-5-RR (ZNF350 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 84121-5-RR

    FC experiment of A431 using 84121-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ZNF350 Recombinant antibody (84121-5-RR, Clone:241225D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84121-5-RR

    Affinity and Kinetic Characterization of 84121-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84121-5-RR against Human ZNF350 were performed. The affinity constant is below 1 pM.

ZNF350 Recombinant antibody

ZNF350 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84121-5-RR

产品说明书

CloneNo. 241225D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

Zinc finger protein ZBRK1, zinc finger protein 350, ZFQR, ZBRK1, KRAB zinc finger protein ZFQR

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84121-5-RR targets ZNF350 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ZNF350 fusion protein Ag23982 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger protein 350
别名 Zinc finger protein ZBRK1, zinc finger protein 350, ZFQR, ZBRK1, KRAB zinc finger protein ZFQR
观测分子量60 kDa
GenBank蛋白编号BC009921
基因名称 ZNF350
Gene ID (NCBI) 59348
RRIDAB_3671683
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9GZX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF350 (Zinc finger protein 350), also known as zinc-finger and BRCA1-interacting protein with a Kruppel-associated box (KRAB) domain (ZBRK1), is involved in the development of several human tumor types, including breast, colon, and cervical carcinomas. ZNF350 has been identified as a transcriptional suppressor that can either form complexes with other proteins or play a direct transcriptional suppressive role in a single-factor form (PMID: 30613364). Overexpression of ZBRK1 has the potential to inhibit cancer cell migration and suppress metastasis activity suggesting that ZBRK1 may behave as a metastasis suppressor (PMID: 19996286).

实验方案

Product Specific Protocols
WB protocol for ZNF350 antibody 84121-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols