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验证数据展示

  • WB analysis of DU 145 using 14072-1-AP

    WB analysis of DU 145 using 14072-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 14072-1-AP (ZNF354A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZNF354A Polyclonal antibody

ZNF354A Polyclonal Antibody for WB, ELISA
Cat No. 14072-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

EZNF, HKL1, KID 1, KID1, TCF 17, TCF17, Transcription factor 17, zinc finger protein 354A, Zinc finger protein eZNF, ZNF354A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14072-1-AP targets ZNF354A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ZNF354A fusion protein Ag5142 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 354A
别名 EZNF, HKL1, KID 1, KID1, TCF 17, TCF17, Transcription factor 17, zinc finger protein 354A, Zinc finger protein eZNF, ZNF354A
计算分子量 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC047105
基因名称 ZNF354A
Gene ID (NCBI) 6940
RRIDAB_2217732
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60765
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF354A, also called EZNF, KID-1 or TCF17, belongs to the Kruppel C2H2-type zinc-finger family of proteins that contain KRAB domains and act as transcriptional regulators. Expressed primarily in the adult kidney, ZNF354A is a transcriptional repressor that plays a role in late renal development and is suppressed after renal ischemia. The N-terminus of ZNF354A contains the KRAB domain which confers transcriptional repressor activity, while the C-terminus contains multiple Cys2His2-zinc fingers. ZNF354A is located in the nucleolus and is thought to specifically influence development of the proximal tubule by shutting off dispensable or inhibitory genes. Reduced ZNF354A expression prevents proper cell differentiation and may, therefore, be implicated in renal carcinoma.

实验方案

Product Specific Protocols
WB protocol for ZNF354A antibody 14072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols