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ZNF354A Polyclonal antibody

ZNF354A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14072-1-AP

Print datasheet

Synonyms

EZNF, HKL1, KID 1, KID1, TCF 17, TCF17, Transcription factor 17, zinc finger protein 354A, Zinc finger protein eZNF, ZNF354A

验证数据展示

  • WB analysis of DU 145 using 14072-1-AP

    WB analysis of DU 145 using 14072-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 14072-1-AP (ZNF354A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inDU 145 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14072-1-AP targets ZNF354A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ZNF354A fusion protein Ag5142 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 354A
Synonyms EZNF, HKL1, KID 1, KID1, TCF 17, TCF17, Transcription factor 17, zinc finger protein 354A, Zinc finger protein eZNF, ZNF354A
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC047105
Gene Symbol ZNF354A
Gene ID (NCBI) 6940
RRIDAB_2217732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60765
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF354A, also called EZNF, KID-1 or TCF17, belongs to the Kruppel C2H2-type zinc-finger family of proteins that contain KRAB domains and act as transcriptional regulators. Expressed primarily in the adult kidney, ZNF354A is a transcriptional repressor that plays a role in late renal development and is suppressed after renal ischemia. The N-terminus of ZNF354A contains the KRAB domain which confers transcriptional repressor activity, while the C-terminus contains multiple Cys2His2-zinc fingers. ZNF354A is located in the nucleolus and is thought to specifically influence development of the proximal tubule by shutting off dispensable or inhibitory genes. Reduced ZNF354A expression prevents proper cell differentiation and may, therefore, be implicated in renal carcinoma.

实验方案

Product Specific Protocols
WB protocol for ZNF354A antibody 14072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols