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验证数据展示

  • WB analysis of HeLa using 26288-1-AP

    WB analysis of HeLa using 26288-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26288-1-AP (ZNF385A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brown disease using 26288-1-AP

    IHC staining of human brown disease using 26288-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26288-1-AP (ZNF385A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brown disease using 26288-1-AP

    IHC staining of human brown disease using 26288-1-AP

    Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26288-1-AP (ZNF385A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HaCaT using 26288-1-AP

    IF Staining of HaCaT using 26288-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using ZNF385A antibody (26288-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

ZNF385A Polyclonal antibody

ZNF385A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26288-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ZNF385, Zinc finger protein 385A, ZFP385

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman brown disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26288-1-AP targets ZNF385A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 ZNF385A fusion protein Ag23577 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 385A
别名 ZNF385, Zinc finger protein 385A, ZFP385
观测分子量 45-50 kDa
GenBank蛋白编号BC029752
基因名称 ZNF385A
Gene ID (NCBI) 25946
RRIDAB_2880464
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PM9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF385A antibody 26288-1-APDownload protocol
IHC protocol for ZNF385A antibody 26288-1-APDownload protocol
IF protocol for ZNF385A antibody 26288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

ZNF385A and ZNF346 Serve as Prognostic Biomarkers Associated with an Inflamed Immunosuppressive Tumor Microenvironment in Hepatocellular Carcinoma

Authors - Qihang Peng
  • KD Validated
View Article
human,mouseWB,IHC

J Proteome Res

Quantitative Proteomics Reveal the Mechanism of MiR-138-5p Suppressing Cervical Cancer via Targeting ZNF385A

Authors - Qihang Peng
  • KD Validated
View Article