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验证数据展示

  • IHC staining of mouse liver using 20799-1-AP

    IHC staining of mouse liver using 20799-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZNF397 antibody included
  • IHC staining of mouse liver using 20799-1-AP

    IHC staining of mouse liver using 20799-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZNF397 antibody included
  • WB analysis of PC-3 using 20799-1-AP

    WB analysis of PC-3 using 20799-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20799-1-AP (ZNF397 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ZNF397 Polyclonal antibody

ZNF397 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20799-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, Rat

应用

WB, IHC, ELISA

别名

zinc finger protein 397, Zinc finger protein 47, ZNF397, ZNF47, ZSCAN15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, mouse testis tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ZNF397 Ready-To-Use IHC Kit. ZNF397 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20799-1-AP targets ZNF397 in WB, IHC, ELISA applications and shows reactivity with human, mouse, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, Rat
免疫原 ZNF397 fusion protein Ag14712 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 397
别名 zinc finger protein 397, Zinc finger protein 47, ZNF397, ZNF47, ZSCAN15
计算分子量 534 aa, 61 kDa
观测分子量 60-63 kDa
GenBank蛋白编号BC006172
基因名称 ZNF397
Gene ID (NCBI) 84307
RRIDAB_2878741
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NF99
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF397 (Zinc Finger Protein 397) is conserved in placental mammals. ZNF397 as a bona fide coactivator of the androgen receptor (AR), essential for the transcriptional program governing AR-driven luminal lineage.

实验方案

Product Specific Protocols
WB protocol for ZNF397 antibody 20799-1-APDownload protocol
IHC protocol for ZNF397 antibody 20799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols