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ZNF397 Polyclonal antibody

ZNF397 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20799-1-AP

Print datasheet

Synonyms

zinc finger protein 397, Zinc finger protein 47, ZNF397, ZNF47, ZSCAN15

验证数据展示

  • IHC staining of mouse liver using 20799-1-AP

    IHC staining of mouse liver using 20799-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZNF397 antibody included
  • IHC staining of mouse liver using 20799-1-AP

    IHC staining of mouse liver using 20799-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20799-1-AP (ZNF397 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZNF397 antibody included
  • WB analysis of PC-3 using 20799-1-AP

    WB analysis of PC-3 using 20799-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20799-1-AP (ZNF397 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells, mouse testis tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ZNF397 Ready-To-Use IHC Kit. ZNF397 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20799-1-AP targets ZNF397 in WB, IHC, ELISA applications and shows reactivity with human, mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, Rat
Immunogen ZNF397 fusion protein Ag14712 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 397
Synonyms zinc finger protein 397, Zinc finger protein 47, ZNF397, ZNF47, ZSCAN15
Calculated Molecular Weight 534 aa, 61 kDa
Observed Molecular Weight 60-63 kDa
GenBank Accession NumberBC006172
Gene Symbol ZNF397
Gene ID (NCBI) 84307
RRIDAB_2878741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NF99
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF397 (Zinc Finger Protein 397) is conserved in placental mammals. ZNF397 as a bona fide coactivator of the androgen receptor (AR), essential for the transcriptional program governing AR-driven luminal lineage.

实验方案

Product Specific Protocols
WB protocol for ZNF397 antibody 20799-1-APDownload protocol
IHC protocol for ZNF397 antibody 20799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols