• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ZNF451 Polyclonal antibody

ZNF451 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25228-1-AP

Print datasheet

Synonyms

KIAA1702, KIAA0576, EC:2.3.2.-, E3 SUMO-protein transferase ZNF451, E3 SUMO-protein ligase ZNF451

验证数据展示

  • IP experiment of HeLa using 25228-1-AP

    IP experiment of HeLa using 25228-1-AP

    IP result of anti-ZNF451 (IP:25228-1-AP, 4ug; Detection:25228-1-AP 1:300) with HeLa cells lysate 1080 ug.

  • WB analysis of HeLa using 25228-1-AP

    WB analysis of HeLa using 25228-1-AP

    WB result of ZNF451 antibody (25228-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZNF451 transfected HeLa cells.

  • WB analysis of HeLa using 25228-1-AP

    WB analysis of HeLa using 25228-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25228-1-AP (ZNF451 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 25228-1-AP

    IHC staining of human placenta using 25228-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25228-1-AP (ZNF451 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 25228-1-AP

    IF Staining of HeLa using 25228-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 25228-1-AP (ZNF451 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25228-1-AP targets ZNF451 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen ZNF451 fusion protein Ag18346 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 451
Synonyms KIAA1702, KIAA0576, EC:2.3.2.-, E3 SUMO-protein transferase ZNF451, E3 SUMO-protein ligase ZNF451
Calculated Molecular Weight 1061 aa, 121 kDa
Observed Molecular Weight 121 kDa
GenBank Accession NumberBC021712
Gene Symbol ZNF451
Gene ID (NCBI) 26036
RRIDAB_2879975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4E5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF451 antibody 25228-1-APDownload protocol
IHC protocol for ZNF451 antibody 25228-1-APDownload protocol
IF protocol for ZNF451 antibody 25228-1-APDownload protocol
IP protocol for ZNF451 antibody 25228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

Changes in SUMO-modified proteins in Epstein-Barr virus infection identifies reciprocal regulation of TRIM24/28/33 complexes and the lytic switch BZLF1

Authors - Carlos F De La Cruz-Herrera
View Article
humanWB,IHC,IF

Cell Rep

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Authors - Yu Zhang
  • KD Validated
View Article
mouseWB

Respir Res

Loss of ZNF451 mediates fibroblast activation and promotes lung fibrosis

Authors - Hong Peng
View Article