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ZNF497 Polyclonal antibody

ZNF497 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25178-1-AP

Print datasheet

Synonyms

zinc finger protein 497, ZNF497

验证数据展示

  • WB analysis of mouse testis using 25178-1-AP

    WB analysis of mouse testis using 25178-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25178-1-AP (ZNF497 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25178-1-AP

    IHC staining of human testis using 25178-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 25178-1-AP

    IHC staining of human kidney using 25178-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25178-1-AP (ZNF497 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25178-1-AP targets ZNF497 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ZNF497 fusion protein Ag18114 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 497
Synonyms zinc finger protein 497, ZNF497
Calculated Molecular Weight 498 aa, 55 kDa
Observed Molecular Weight 61-65 kDa
GenBank Accession NumberBC118983
Gene Symbol ZNF497
Gene ID (NCBI) 162968
RRIDAB_2879944
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZNH5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF497 antibody 25178-1-APDownload protocol
IHC protocol for ZNF497 antibody 25178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols