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验证数据展示

  • WB analysis using 23716-1-AP

    WB analysis using 23716-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23716-1-AP (ZNF521 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 23716-1-AP

    IF Staining of A431 using 23716-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZNF521 antibody (23716-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

ZNF521 Polyclonal antibody

ZNF521 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23716-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Zfp521, zinc finger protein 521, LYST interacting protein 3, LIP3, Evi3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuH-7 cells, L02 cells, T2 cells, THP-1 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23716-1-AP targets ZNF521 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZNF521 fusion protein Ag20540 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 521
别名 Zfp521, zinc finger protein 521, LYST interacting protein 3, LIP3, Evi3
计算分子量 1311 aa, 148 kDa
观测分子量190 kDa
GenBank蛋白编号BC113648
基因名称 ZNF521
Gene ID (NCBI) 25925
RRIDAB_3669415
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96K83
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger protein 521 (EHZF/ZNF521) is a transcription co-factor originally identified for its abundant and selective expression in early progenitors of the human hematopoietic system. In the hematopoietic system ZNF521 is highly expressed in stem and progenitor cells but not in more differentiated precursors or mature leukocytes (PMID: 23907569, 21593590).

实验方案

Product Specific Protocols
WB protocol for ZNF521 antibody 23716-1-APDownload protocol
IF protocol for ZNF521 antibody 23716-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols