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验证数据展示

  • WB analysis of SH-SY5Y using 25592-1-AP

    WB analysis of SH-SY5Y using 25592-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25592-1-AP (ZNF549 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 25592-1-AP

    IF Staining of A549 using 25592-1-AP

    Immunofluorescent analysis of A549 cells using 25592-1-AP (ZNF549 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ZNF549 Polyclonal antibody

ZNF549 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25592-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

zinc finger protein 549, ZNF549

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, A549 cells, HeLa cells, SMMC-7721 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25592-1-AP targets ZNF549 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZNF549 fusion protein Ag22371 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 549
别名 zinc finger protein 549, ZNF549
计算分子量 640 aa, 74 kDa
观测分子量 74 kDa
GenBank蛋白编号BC060863
基因名称 ZNF549
Gene ID (NCBI) 256051
RRIDAB_2880147
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P9A3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF549 antibody 25592-1-APDownload protocol
IF protocol for ZNF549 antibody 25592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols