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验证数据展示

  • WB analysis of MCF-7 using 25302-1-AP

    WB analysis of MCF-7 using 25302-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25302-1-AP (ZNF571 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 25302-1-AP

    IP experiment of MCF-7 using 25302-1-AP

    IP result of anti-ZNF571 (IP:25302-1-AP, 4ug; Detection:25302-1-AP 1:300) with MCF-7 cells lysate 1200ug.

  • IHC staining of human testis using 25302-1-AP

    IHC staining of human testis using 25302-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25302-1-AP (ZNF571 Antibody) at dilution of 1:50 (under 40x lens).

ZNF571 Polyclonal antibody

ZNF571 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 25302-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

HSPC059, zinc finger protein 571, ZNF571

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25302-1-AP targets ZNF571 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human
免疫原 ZNF571 fusion protein Ag18151 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 571
别名 HSPC059, zinc finger protein 571, ZNF571
计算分子量 609 aa, 71 kDa
观测分子量 65 kDa
GenBank蛋白编号BC114479
基因名称 ZNF571
Gene ID (NCBI) 51276
RRIDAB_2880019
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z3V5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF571 antibody 25302-1-APDownload protocol
IHC protocol for ZNF571 antibody 25302-1-APDownload protocol
IP protocol for ZNF571 antibody 25302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols