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ZNF571 Polyclonal antibody

ZNF571 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25302-1-AP

Print datasheet

Synonyms

HSPC059, zinc finger protein 571, ZNF571

验证数据展示

  • WB analysis of MCF-7 using 25302-1-AP

    WB analysis of MCF-7 using 25302-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25302-1-AP (ZNF571 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 25302-1-AP

    IP experiment of MCF-7 using 25302-1-AP

    IP result of anti-ZNF571 (IP:25302-1-AP, 4ug; Detection:25302-1-AP 1:300) with MCF-7 cells lysate 1200ug.

  • IHC staining of human testis using 25302-1-AP

    IHC staining of human testis using 25302-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25302-1-AP (ZNF571 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25302-1-AP is ZNF571 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ZNF571 fusion protein Ag18151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 571
Synonyms HSPC059, zinc finger protein 571, ZNF571
Calculated Molecular Weight 609 aa, 71 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC114479
Gene Symbol ZNF571
Gene ID (NCBI) 51276
RRIDAB_2880019
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3V5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF571 antibody 25302-1-APDownload protocol
IHC protocol for ZNF571 antibody 25302-1-APDownload protocol
IP protocol for ZNF571 antibody 25302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols