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ZNF597 Polyclonal antibody

ZNF597 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20922-1-AP

Print datasheet

Synonyms

zinc finger protein 597, ZNF597

验证数据展示

  • WB analysis of Jurkat using 20922-1-AP

    WB analysis of Jurkat using 20922-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20922-1-AP (ZNF597 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20922-1-AP

    IHC staining of human brain using 20922-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20922-1-AP (ZNF597 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 20922-1-AP

    IHC staining of human brain using 20922-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20922-1-AP (ZNF597 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20922-1-AP is ZNF597 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ZNF597 fusion protein Ag15183 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 597
Synonyms zinc finger protein 597, ZNF597
Calculated Molecular Weight 424 aa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC029899
Gene Symbol ZNF597
Gene ID (NCBI) 146434
RRIDAB_10859828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96LX8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF597 antibody 20922-1-APDownload protocol
IHC protocol for ZNF597 antibody 20922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols