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验证数据展示

  • WB analysis using 31972-1-AP

    WB analysis using 31972-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31972-1-AP (ZNF618 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 31972-1-AP

    IHC staining of human stomach cancer using 31972-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cervical cancer using 31972-1-AP

    IHC staining of human cervical cancer using 31972-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 31972-1-AP (ZNF618 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 31972-1-AP

    IF Staining of A549 using 31972-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ZNF618 antibody (31972-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

ZNF618 Polyclonal antibody

ZNF618 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31972-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Zinc finger protein 618, KIAA1952

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, SH-SY5Y cells, Caki-2 cells
Positive IHC detected inhuman cervical cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31972-1-AP targets ZNF618 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZNF618 fusion protein Ag36487 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 618
别名 Zinc finger protein 618, KIAA1952
计算分子量105kDa，954aa
观测分子量100-113 kDa
GenBank蛋白编号NM_001318042.1
基因名称 ZNF618
Gene ID (NCBI) 114991
RRIDAB_3670163
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5T7W0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells.

实验方案

Product Specific Protocols
WB protocol for ZNF618 antibody 31972-1-APDownload protocol
IHC protocol for ZNF618 antibody 31972-1-APDownload protocol
IF protocol for ZNF618 antibody 31972-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols