验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, MCF-7 cells, Caki-2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84998-1-RR targets ZNF618 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ZNF618 fusion protein Ag36487 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 618 |
| 别名 | 242566D9, KIAA1952, Zinc finger protein 618 |
| 计算分子量 | 105kDa，954aa |
| 观测分子量 | 110-130 kDa |
| GenBank蛋白编号 | NM_001318042.1 |
| 基因名称 | ZNF618 |
| Gene ID (NCBI) | 114991 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q5T7W0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF618 antibody 84998-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |