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验证数据展示

  • WB analysis of HeLa using 20804-1-AP

    WB analysis of HeLa using 20804-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20804-1-AP (ZNF622 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 20804-1-AP

    IHC staining of human hepatocirrhosis using 20804-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20804-1-AP (ZNF622 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 20804-1-AP

    IHC staining of human hepatocirrhosis using 20804-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20804-1-AP (ZNF622 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 20804-1-AP

    IF Staining of HeLa using 20804-1-AP

    Immunofluorescent analysis of Hela cells, using ZNF622 antibody 20804-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

ZNF622 Polyclonal antibody

ZNF622 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20804-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Zinc finger like protein 9, zinc finger protein 622, ZNF622, ZPR9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, human brain tissue, mouse lung tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20804-1-AP targets ZNF622 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ZNF622 fusion protein Ag14741 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 622
别名 Zinc finger like protein 9, zinc finger protein 622, ZNF622, ZPR9
计算分子量 477 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC008752
基因名称 ZNF622
Gene ID (NCBI) 90441
RRIDAB_10693628
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ969S3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF622 antibody 20804-1-APDownload protocol
IHC protocol for ZNF622 antibody 20804-1-APDownload protocol
IF protocol for ZNF622 antibody 20804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols