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ZNF645 Polyclonal antibody

ZNF645 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25600-1-AP

Print datasheet

Synonyms

HAKAIL, zinc finger protein 645, ZNF645

验证数据展示

  • IHC staining of human prostate cancer using 25600-1-AP

    IHC staining of human prostate cancer using 25600-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25600-1-AP (ZNF645 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 25600-1-AP

    IHC staining of human prostate cancer using 25600-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25600-1-AP (ZNF645 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human testis using 25600-1-AP

    WB analysis of human testis using 25600-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 25600-1-AP (ZNF645 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25600-1-AP is ZNF645 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ZNF645 fusion protein Ag22346 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 645
Synonyms HAKAIL, zinc finger protein 645, ZNF645
Calculated Molecular Weight 425 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC126190
Gene Symbol ZNF645
Gene ID (NCBI) 158506
RRIDAB_2880153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N7E2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF645 antibody 25600-1-APDownload protocol
IHC protocol for ZNF645 antibody 25600-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols