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验证数据展示

  • WB analysis using 84624-4-RR

    WB analysis using 84624-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84624-4-RR (ZNF649 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84624-4-RR

    Affinity and Kinetic Characterization of 84624-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84624-4-RR against Human ZNF649 were performed. The affinity constant is 4.71 nM.

ZNF649 Recombinant antibody

ZNF649 Recombinant Antibody for WB, ELISA
Cat No. 84624-4-RR

产品说明书

CloneNo. 242036C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Zinc finger protein 649, 242036C7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HCT116 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84624-4-RR targets ZNF649 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ZNF649 fusion protein Ag22700 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger protein 649
别名 Zinc finger protein 649, 242036C7
计算分子量 505 aa, 58 kDa
观测分子量50 kDa
GenBank蛋白编号BC005368
基因名称 ZNF649
Gene ID (NCBI) 65251
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9BS31
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF649, also named as Zinc finger protein 649, is a 505 amino acid protein, which contains The one KRAB domain and belongs to the krueppel C2H2-type zinc-finger protein family. ZNF649 is highly expressed in heart, skeletal muscle, and brain and lower expression in liver, lung, kidney, pancreas and placenta. ZNF649 as a regulator of transcriptional factor complexes may suppress SRE and AP-1 transcription activities by growth factor signaling pathways.

实验方案

Product Specific Protocols
WB protocol for ZNF649 antibody 84624-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols