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验证数据展示

  • WB analysis using 21329-1-AP

    WB analysis using 21329-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21329-1-AP (ZNF671 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 21329-1-AP

    IF Staining of U-251 using 21329-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ZNF671 antibody (21329-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

ZNF671 Polyclonal antibody

ZNF671 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21329-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Zinc finger protein 671

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, MCF-7 cells, MDA-MB-231 cells, SKOV-3 cells, U-251 cells
Positive IF/ICC detected inU-251 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21329-1-AP targets ZNF671 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 ZNF671 fusion protein Ag15319 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 671
别名 Zinc finger protein 671
计算分子量 556 aa, 63 kDa
观测分子量 61-70 kDa
GenBank蛋白编号BC025728
基因名称 ZNF671
Gene ID (NCBI) 79891
RRIDAB_10755298
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAW3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF671 antibody 21329-1-APDownload protocol
IF protocol for ZNF671 antibody 21329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Oncol

The Tumor Suppressor Role of Zinc Finger Protein 671 (ZNF671) in Multiple Tumors Based on Cancer Single-Cell Sequencing.

Authors - Jian Zhang
View Article
humanWB

Front Oncol

Epigenetic-Mediated Downregulation of Zinc Finger Protein 671 (ZNF671) Predicts Poor Prognosis in Multiple Solid Tumors.

Authors - Jian Zhang
View Article
humanWB

J Exp Clin Cancer Res

Epigenetic mediated zinc finger protein 671 downregulation promotes cell proliferation and tumorigenicity in nasopharyngeal carcinoma by inhibiting cell cycle arrest.

Authors - Jian Zhang
  • KD Validated
View Article
humanWB

Int J Biol Sci

Increased methylation of ZNF671 suppresses tumor progression by promoting MAPK6 transcription in laryngeal carcinoma

Authors - Wei Zhang
View Article