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ZNF74 Polyclonal antibody

ZNF74 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21843-1-AP

Print datasheet

Synonyms

Cos52, hZNF7, Zfp520, zinc finger protein 74, ZNF520, ZNF74

验证数据展示

  • WB analysis of Jurkat using 21843-1-AP

    WB analysis of Jurkat using 21843-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21843-1-AP (ZNF74 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 21843-1-AP

    IF Staining of HepG2 using 21843-1-AP

    Immunofluorescent analysis of HepG2 cells, using ZNF74 antibody 21843-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inJurkat cells, human brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 21843-1-AP is ZNF74 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ZNF74 fusion protein Ag13581 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 74
Synonyms Cos52, hZNF7, Zfp520, zinc finger protein 74, ZNF520, ZNF74
Calculated Molecular Weight 644 aa, 72 kDa
Observed Molecular Weight 64-72 kDa
GenBank Accession NumberBC013395
Gene Symbol ZNF74
Gene ID (NCBI) 7625
RRIDAB_10836937
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16587
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF74 antibody 21843-1-APDownload protocol
IF protocol for ZNF74 antibody 21843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomolecules

5'-tRF-19-Q1Q89PJZ Suppresses the Proliferation and Metastasis of Pancreatic Cancer Cells via Regulating Hexokinase 1-Mediated Glycolysis

Authors - Wenpeng Cao
View Article