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验证数据展示

  • WB analysis of mouse brain using 25411-1-AP

    WB analysis of mouse brain using 25411-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 25411-1-AP

    IP experiment of mouse brain using 25411-1-AP

    IP result of anti-ZNF740 (IP:25411-1-AP, 3ug; Detection:25411-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • IHC staining of human ovary cancer using 25411-1-AP

    IHC staining of human ovary cancer using 25411-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25411-1-AP (ZNF740 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZNF740 Polyclonal antibody

ZNF740 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 25411-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA and More (1)

别名

Zinc finger protein 740, Zfp740, TB7, OriLyt TD-element-binding protein 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue, PC-3 cells, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25411-1-AP targets ZNF740 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, ChIP
经测试反应性 human, mouse
文献引用反应性human
免疫原 ZNF740 fusion protein Ag22295 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 740
别名 Zinc finger protein 740, Zfp740, TB7, OriLyt TD-element-binding protein 7
计算分子量 193 aa, 22 kDa
观测分子量 22-30 kDa
GenBank蛋白编号BC053557
基因名称 ZNF740
Gene ID (NCBI) 283337
RRIDAB_2880065
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NDX6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF740 antibody 25411-1-APDownload protocol
IHC protocol for ZNF740 antibody 25411-1-APDownload protocol
IP protocol for ZNF740 antibody 25411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator

Authors - Lixia Zhang
  • KD Validated
View Article
humanWB,IHC,ChIP

Int J Oncol

ZNF740 facilitates the malignant progression of hepatocellular carcinoma via the METTL3/HIF‑1A signaling axis

Authors - Hao Zhang
View Article