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ZNF740 Polyclonal antibody

ZNF740 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25411-1-AP

Print datasheet

Synonyms

Zinc finger protein 740, Zfp740, TB7, OriLyt TD-element-binding protein 7

验证数据展示

  • WB analysis of mouse brain using 25411-1-AP

    WB analysis of mouse brain using 25411-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25411-1-AP (ZNF740 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 25411-1-AP

    IP experiment of mouse brain using 25411-1-AP

    IP result of anti-ZNF740 (IP:25411-1-AP, 3ug; Detection:25411-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • IHC staining of human ovary cancer using 25411-1-AP

    IHC staining of human ovary cancer using 25411-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25411-1-AP (ZNF740 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue, PC-3 cells, mouse liver tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25411-1-AP targets ZNF740 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, ChIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ZNF740 fusion protein Ag22295 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 740
Synonyms Zinc finger protein 740, Zfp740, TB7, OriLyt TD-element-binding protein 7
Calculated Molecular Weight 193 aa, 22 kDa
Observed Molecular Weight 22-30 kDa
GenBank Accession NumberBC053557
Gene Symbol ZNF740
Gene ID (NCBI) 283337
RRIDAB_2880065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NDX6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF740 antibody 25411-1-APDownload protocol
IHC protocol for ZNF740 antibody 25411-1-APDownload protocol
IP protocol for ZNF740 antibody 25411-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Death Dis

CRISPR screen of venetoclax response-associated genes identifies transcription factor ZNF740 as a key functional regulator

Authors - Lixia Zhang
  • KD Validated
View Article
humanWB,IHC,ChIP

Int J Oncol

ZNF740 facilitates the malignant progression of hepatocellular carcinoma via the METTL3/HIF‑1A signaling axis

Authors - Hao Zhang
View Article