验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24543-1-AP targets ZNF746 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ZNF746 fusion protein Ag21566 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 746 |
| 别名 | Zinc finger protein 746, Parkin-interacting substrate, PARIS, FLJ31413 |
| 计算分子量 | 644 aa, 69 kDa |
| 观测分子量 | 85-95 kDa |
| GenBank蛋白编号 | BC068505 |
| 基因名称 | ZNF746 |
| Gene ID (NCBI) | 155061 |
| RRID | AB_2879598 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6NUN9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN (PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF746 antibody 24543-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Neuronal NLRP3 is a parkin substrate that drives neurodegeneration in Parkinson's disease.
| ||
Sci Adv Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease. | ||
Brain Parkin interacting substrate zinc finger protein 746 is a pathological mediator in Parkinson's disease. | ||
Brain Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration. | ||
Front Aging Neurosci Identification and evaluation of midbrain specific longevity-related genes in exceptionally long-lived but healthy mice | ||
Biochem Biophys Res Commun ZNF746/PARIS promotes the occurrence of hepatocellular carcinoma.
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