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  • KD/KO Validated

ZNF746 Polyclonal antibody

ZNF746 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 24543-1-AP

Print datasheet

Synonyms

Zinc finger protein 746, Parkin-interacting substrate, PARIS, FLJ31413



经过测试的应用

Positive WB detected inHEK-293 cells, HEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy ZNF746 Ready-To-Use IHC Kit. ZNF746 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24543-1-AP targets ZNF746 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen ZNF746 fusion protein Ag21566 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 746
Synonyms Zinc finger protein 746, Parkin-interacting substrate, PARIS, FLJ31413
Calculated Molecular Weight 644 aa, 69 kDa
Observed Molecular Weight 85-95 kDa
GenBank Accession NumberBC068505
Gene Symbol ZNF746
Gene ID (NCBI) 155061
RRIDAB_2879598
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NUN9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN (PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).

实验方案

Product Specific Protocols
WB protocol for ZNF746 antibody 24543-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neuron

Neuronal NLRP3 is a parkin substrate that drives neurodegeneration in Parkinson's disease.

Authors - Nikhil Panicker
  • KO Validated
human,mouseWB

Sci Adv

Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease.

Authors - Sheila K Pirooznia
mouseWB

Brain

Parkin interacting substrate zinc finger protein 746 is a pathological mediator in Parkinson's disease.

Authors - Saurav Brahmachari
human,mouseWB,IF

Brain

Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.

Authors - Hyojung Kim
mouseWB

Front Aging Neurosci

Identification and evaluation of midbrain specific longevity-related genes in exceptionally long-lived but healthy mice

Authors - Hyojung Kim
human,mouseWB,IHC

Biochem Biophys Res Commun

ZNF746/PARIS promotes the occurrence of hepatocellular carcinoma.

Authors - Hanna Kim
  • KO Validated