验证数据展示
经过测试的应用
| Positive IP detected in | SiHa cells |
| Positive IHC detected in | human oesophagus cancer tissue, human skin tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
21752-1-AP targets ZNF750 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ZNF750 fusion protein Ag16116 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 750 |
| 别名 | Protein ZNF750, Zfp750, Zinc finger protein 750 |
| 计算分子量 | 723 aa, 77 kDa |
| 观测分子量 | 70-77 kDa |
| GenBank蛋白编号 | BC109037 |
| 基因名称 | ZNF750 |
| Gene ID (NCBI) | 79755 |
| RRID | AB_10863125 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q32MQ0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZNF750 is a novel nuclear protein highly expressed in differentiated skin keratinocytes. Mutation of ZNF750 is linked with familial psoriasis. Recently ZNF was found to be significantly mutated in esophageal squamous cell carcinoma (ESCC). Expression of ZNF750 is decreased in ESCC compared to the normal tissue.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ZNF750 antibody 21752-1-AP | Download protocol |
| IP protocol for ZNF750 antibody 21752-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Cancer Identification of calmodulin-like protein 5 as tumor-suppressor gene silenced during early stage of carcinogenesis in squamous cell carcinoma of uterine cervix. | ||
Oncol Lett Differentiation-related zinc finger protein 750 suppresses cell growth in esophageal squamous cell carcinoma. | ||
Cell Death Discov TRIM29 hypermethylation drives esophageal cancer progression via suppression of ZNF750 | ||
Cell Death Dis GPR37-enhanced ubiquitination of ATP1A1 inhibits tumor progression and radiation resistance in esophageal squamous cell carcinoma |
