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验证数据展示

  • WB analysis of HepG2 using 25449-1-AP

    WB analysis of HepG2 using 25449-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25449-1-AP (ZNF774 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 25449-1-AP

    IP experiment of HepG2 using 25449-1-AP

    IP result of anti-ZNF774 (IP:25449-1-AP, 3ug; Detection:25449-1-AP 1:600) with HepG2 cells lysate 2400ug.

ZNF774 Polyclonal antibody

ZNF774 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25449-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

zinc finger protein 774, ZNF774

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25449-1-AP targets ZNF774 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 ZNF774 fusion protein Ag22300 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 774
别名 zinc finger protein 774, ZNF774
计算分子量 483 aa, 55 kDa
观测分子量 55-63 kDa
GenBank蛋白编号BC067279
基因名称 ZNF774
Gene ID (NCBI) 342132
RRIDAB_2880086
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6NX45
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF774 antibody 25449-1-APDownload protocol
IP protocol for ZNF774 antibody 25449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols