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ZNF774 Polyclonal antibody

ZNF774 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25449-1-AP

Print datasheet

Synonyms

zinc finger protein 774, ZNF774

验证数据展示

  • WB analysis of HepG2 using 25449-1-AP

    WB analysis of HepG2 using 25449-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25449-1-AP (ZNF774 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 25449-1-AP

    IP experiment of HepG2 using 25449-1-AP

    IP result of anti-ZNF774 (IP:25449-1-AP, 3ug; Detection:25449-1-AP 1:600) with HepG2 cells lysate 2400ug.

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25449-1-AP is ZNF774 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen ZNF774 fusion protein Ag22300 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 774
Synonyms zinc finger protein 774, ZNF774
Calculated Molecular Weight 483 aa, 55 kDa
Observed Molecular Weight 55-63 kDa
GenBank Accession NumberBC067279
Gene Symbol ZNF774
Gene ID (NCBI) 342132
RRIDAB_2880086
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NX45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF774 antibody 25449-1-APDownload protocol
IP protocol for ZNF774 antibody 25449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols