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ZNF8 Polyclonal antibody

ZNF8 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 17448-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 17448-1-AP

    WB analysis using 17448-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17448-1-AP (ZNF8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 17448-1-AP

    IHC staining of mouse cerebellum using 17448-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17448-1-AP (ZNF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 17448-1-AP

    IF Staining of mouse cerebellum using 17448-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 17448-1-AP

    IF Staining of mouse cerebellum using 17448-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using ZNF8 antibody (17448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17448-1-AP targets ZNF8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ZNF8 fusion protein Ag11571 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 8
Synonyms
Calculated Molecular Weight 575 aa, 65 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC039323
Gene Symbol ZNF8
Gene ID (NCBI) 7554
RRIDAB_3669275
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17098
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZNF8 is a transcriptional repressor. It may modulate BMP and TGF-beta signal transduction, through its interaction with SMAD proteins.

实验方案

Product Specific Protocols
IHC protocol for ZNF8 antibody 17448-1-APDownload protocol
IF protocol for ZNF8 antibody 17448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols