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ZNF839 Polyclonal antibody

ZNF839 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20539-1-AP

Print datasheet

Synonyms

Zinc finger protein 839, Renal carcinoma antigen NY-REN-50, C14orf131

验证数据展示

  • WB analysis of HeLa using 20539-1-AP

    WB analysis of HeLa using 20539-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20539-1-AP (ZNF839 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 20539-1-AP

    IHC staining of human renal cell carcinoma using 20539-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 20539-1-AP (ZNF839 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human renal cell carcinoma using 20539-1-AP

    IHC staining of human renal cell carcinoma using 20539-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 20539-1-AP (ZNF839 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20539-1-AP

    IF Staining of HepG2 using 20539-1-AP

    Immunofluorescent analysis of HepG2 cells, using ZNF839 antibody 20539-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, human small intestine tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20539-1-AP targets ZNF839 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ZNF839 fusion protein Ag14573 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 839
Synonyms Zinc finger protein 839, Renal carcinoma antigen NY-REN-50, C14orf131
Calculated Molecular Weight 927 aa, 99 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC006222
Gene Symbol ZNF839
Gene ID (NCBI) 55778
RRIDAB_10695759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA8K0R7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF839 antibody 20539-1-APDownload protocol
IHC protocol for ZNF839 antibody 20539-1-APDownload protocol
IF protocol for ZNF839 antibody 20539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols