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验证数据展示

  • WB analysis of HeLa using 20539-1-AP

    WB analysis of HeLa using 20539-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20539-1-AP (ZNF839 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 20539-1-AP

    IHC staining of human renal cell carcinoma using 20539-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 20539-1-AP (ZNF839 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human renal cell carcinoma using 20539-1-AP

    IHC staining of human renal cell carcinoma using 20539-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 20539-1-AP (ZNF839 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 20539-1-AP

    IF Staining of HepG2 using 20539-1-AP

    Immunofluorescent analysis of HepG2 cells, using ZNF839 antibody 20539-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

ZNF839 Polyclonal antibody

ZNF839 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20539-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Zinc finger protein 839, Renal carcinoma antigen NY-REN-50, C14orf131

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human small intestine tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20539-1-AP targets ZNF839 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZNF839 fusion protein Ag14573 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger protein 839
别名 Zinc finger protein 839, Renal carcinoma antigen NY-REN-50, C14orf131
计算分子量 927 aa, 99 kDa
观测分子量 87 kDa
GenBank蛋白编号BC006222
基因名称 ZNF839
Gene ID (NCBI) 55778
RRIDAB_10695759
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA8K0R7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZNF839 antibody 20539-1-APDownload protocol
IHC protocol for ZNF839 antibody 20539-1-APDownload protocol
IF protocol for ZNF839 antibody 20539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols