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验证数据展示

  • WB analysis using 67387-1-Ig

    WB analysis using 67387-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67387-1-Ig (ZO-2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67387-1-Ig

    WB analysis of HeLa using 67387-1-Ig

    WB result of ZO-2 antibody (67387-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZO-2 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

ZO-2 Monoclonal antibody

ZO-2 Monoclonal Antibody for WB, ELISA
Cat No. 67387-1-Ig

产品说明书

CloneNo. 1D2E5

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Rat

应用

WB, ELISA

别名

Tight junction protein 2, Tight junction protein ZO 2, TJP2, X104, ZO 2, ZO2, ZO-2, Zona occludens protein 2, Zonula occludens protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, LNCaP cells, PC-3 cells, HepG2 cells, HEK-293 cells, HSC-T6 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67387-1-Ig targets ZO-2 in WB, ELISA applications and shows reactivity with Human, Rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Rat
免疫原 ZO-2 fusion protein Ag13577 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 tight junction protein 2 (zona occludens 2)
别名 Tight junction protein 2, Tight junction protein ZO 2, TJP2, X104, ZO 2, ZO2, ZO-2, Zona occludens protein 2, Zonula occludens protein 2
计算分子量 134 kDa
观测分子量 150 kDa
GenBank蛋白编号BC027592
基因名称 TJP2
Gene ID (NCBI) 9414
RRIDAB_2882631
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UDY2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZO-2 antibody 67387-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols