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  • Featured Product
  • KD/KO Validated

ZO-2 Recombinant antibody, PBS Only

ZO-2 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230474D1

Cat no : 83111-1-PBS

Print datasheet

Synonyms

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

验证数据展示

  • WB analysis using 83111-1-RR (same clone as 83111-1-PBS)

    WB analysis using 83111-1-RR (same clone as 83111-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83111-1-RR (ZO-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 83111-1-RR (same clone as 83111-1-PBS)

    WB analysis of HeLa using 83111-1-RR (same clone as 83111-1-PBS)

    WB result of ZO-2 antibody (83111-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZO-2 transfected HeLa cells. This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 83111-1-RR (same clone as 83111-1-PBS)

    IHC staining of mouse colon using 83111-1-RR (same clone as 83111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 83111-1-RR (same clone as 83111-1-PBS)

    IHC staining of mouse colon using 83111-1-RR (same clone as 83111-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83111-1-RR (ZO-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83111-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83111-1-RR

    Affinity and Kinetic Characterization of 83111-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83111-1-RR against Human ZO-2 were performed. The affinity constant is 30.8 pM.

产品信息

83111-1-PBS targets ZO-2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ZO-2 fusion protein Ag13534 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tight junction protein 2 (zona occludens 2)
Synonyms TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2
Calculated Molecular Weight 134 kDa
Observed Molecular Weight ~150 kDa
GenBank Accession NumberBC027592
Gene Symbol TJP2
Gene ID (NCBI) 9414
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UDY2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.