ZO-1 Polyclonal antibody
ZO-1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HT-29 cells, HUVEC cells, HepG2 cells |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse heart tissue |
Positive IF/ICC detected in | MCF-7 cells |
Freshly prepared samples are recommended.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IF | See 1 publications below |
产品信息
30487-1-AP targets ZO-1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | ZO-1 fusion protein Ag33182 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | tight junction protein 1 (zona occludens 1) |
Synonyms | TJP1, ZO1, ZO 1, Tight junction protein ZO-1, Tight junction protein ZO 1 |
Calculated Molecular Weight | 1748 aa, 195 kDa |
Observed Molecular Weight | 230 kDa |
GenBank Accession Number | BC111712 |
Gene Symbol | ZO-1 |
Gene ID (NCBI) | 7082 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q07157 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ZO-1 antibody 30487-1-AP | Download protocol |
IHC protocol for ZO-1 antibody 30487-1-AP | Download protocol |
IF protocol for ZO-1 antibody 30487-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Med 5α-Hydroxycostic acid inhibits choroidal neovascularization in rats through a dual signalling pathway mediated by VEGF and angiopoietin 2 | ||
Cells The Natural Product Secoemestrin C Inhibits Colorectal Cancer Stem Cells via p38-S100A8 Feed-Forward Regulatory Loop | ||
Biomed Pharmacother Disruption of blood-brain barrier and endothelial-to-mesenchymal transition are attenuated by Astragalus polysaccharides mediated through upregulation of ETS1 expression in experimental autoimmune encephalomyelitis |