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验证数据展示

  • WB analysis using 21832-1-AP

    WB analysis using 21832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21832-1-AP (ZP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse ovary using 21832-1-AP

    IF Staining of mouse ovary using 21832-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP2 antibody (21832-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ZP2 Polyclonal antibody

ZP2 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 21832-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA

别名

ZPA, Zp-2, Zp 2, Zona pellucida sperm-binding protein 2, Processed zona pellucida sperm-binding protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSKOV-3 cells, mouse brain tissue, mouse ovary tissue, rat brain tissue
Positive IF-P detected inmouse ovary tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21832-1-AP targets ZP2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ZP2 fusion protein Ag16386 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zona pellucida glycoprotein 2 (sperm receptor)
别名 ZPA, Zp-2, Zp 2, Zona pellucida sperm-binding protein 2, Processed zona pellucida sperm-binding protein 2
计算分子量 745 aa, 82 kDa
观测分子量75-82 kDa
GenBank蛋白编号BC096304
基因名称 ZP2
Gene ID (NCBI) 7783
RRIDAB_3669375
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05996
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZP2 is a major component of the zona pellucida to which sperm bind prior to penetrating the zona matrix and fusing with the egg. Following fertilization and cortical granule exocytosis, ZP2 is cleaved by ovastacin after which sperm do not bind to the zona pellucida. The zona pellucida and ovastacin proteins transverse the endomembrane system during oogenesis during which passage ZP2 remains intact. Secreted ZP2 is incorporated into the extracellular zona pellucida surrounding ovulated eggs and is cleaved in the zona matrix surrounding 2-cell embryos. (PMID: 28114310)

实验方案

Product Specific Protocols
WB protocol for ZP2 antibody 21832-1-APDownload protocol
IF protocol for ZP2 antibody 21832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols