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ZP2 Polyclonal antibody

ZP2 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 21832-1-AP

Print datasheet

Synonyms

ZPA, Zp-2, Zp 2, Zona pellucida sperm-binding protein 2, Processed zona pellucida sperm-binding protein 2

验证数据展示

  • WB analysis using 21832-1-AP

    WB analysis using 21832-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21832-1-AP (ZP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse ovary using 21832-1-AP

    IF Staining of mouse ovary using 21832-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP2 antibody (21832-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSKOV-3 cells, mouse brain tissue, mouse ovary tissue, rat brain tissue
Positive IF-P detected inmouse ovary tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21832-1-AP targets ZP2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ZP2 fusion protein Ag16386 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zona pellucida glycoprotein 2 (sperm receptor)
Synonyms ZPA, Zp-2, Zp 2, Zona pellucida sperm-binding protein 2, Processed zona pellucida sperm-binding protein 2
Calculated Molecular Weight 745 aa, 82 kDa
Observed Molecular Weight75-82 kDa
GenBank Accession NumberBC096304
Gene Symbol ZP2
Gene ID (NCBI) 7783
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05996
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZP2 is a major component of the zona pellucida to which sperm bind prior to penetrating the zona matrix and fusing with the egg. Following fertilization and cortical granule exocytosis, ZP2 is cleaved by ovastacin after which sperm do not bind to the zona pellucida. The zona pellucida and ovastacin proteins transverse the endomembrane system during oogenesis during which passage ZP2 remains intact. Secreted ZP2 is incorporated into the extracellular zona pellucida surrounding ovulated eggs and is cleaved in the zona matrix surrounding 2-cell embryos. (PMID: 28114310)

实验方案

Product Specific Protocols
WB protocol for ZP2 antibody 21832-1-APDownload protocol
IF protocol for ZP2 antibody 21832-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols