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验证数据展示

  • WB analysis using 85084-1-RR (same clone as 85084-1-PBS)

    WB analysis using 85084-1-RR (same clone as 85084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85084-1-RR (ZP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

    IF Staining of mouse ovary using 85084-1-RR (same clone as 85084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP3 antibody (85084-1-RR, Clone: 242729A8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85084-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01801-2

    Cytometric bead array standard curve of MP01801-2

    Cytometric bead array standard curve of MP01801-2, ZP3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85084-1-PBS. Detection antibody: 85084-2-PBS. Standard: Ag15791. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85084-1-RR

    Affinity and Kinetic Characterization of 85084-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85084-1-RR against Human ZP3 were performed. The affinity constant is below 1 pM.

ZP3 Recombinant antibody, PBS Only (Capture)

ZP3 Uni-rAbTM Recombinant Antibody for WB, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 85084-1-PBS

产品说明书

CloneNo. 242729A8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, Cytometric bead array, Indirect ELISA

别名

242729A8, Processed zona pellucida sperm-binding protein 3, Zona pellucida sperm-binding protein 3, Zp 3, Zp-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

85084-1-PBS targets ZP3 as part of a matched antibody pair:

MP01801-2: 85084-1-PBS capture and 85084-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 ZP3 fusion protein Ag15791 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zona pellucida glycoprotein 3 (sperm receptor)
别名 242729A8, Processed zona pellucida sperm-binding protein 3, Zona pellucida sperm-binding protein 3, Zp 3, Zp-3
计算分子量 373 aa, 41 kDa
观测分子量47 kDa
GenBank蛋白编号BC113949
基因名称 ZP3
Gene ID (NCBI) 7784
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21754
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that is processed into mature glycoprotein with larger apparent molecular mass (PMID: 8588935; 10341000).