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ZPBP Polyclonal antibody

ZPBP Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, ELISA

Conjugate

Unconjugated

Cat no : 15835-1-AP

Print datasheet

Synonyms

SP38, zona pellucida binding protein, ZPBP, ZPBP1

验证数据展示

  • IP experiment of mouse testis using 15835-1-AP

    IP experiment of mouse testis using 15835-1-AP

    IP result of anti-ZPBP (IP:15835-1-AP, 3ug; Detection:15835-1-AP 1:300) with mouse testis tissue lysate 2800ug.

经过测试的应用

Positive IP detected inmouse testis tissue

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15835-1-AP targets ZPBP in IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ZPBP fusion protein Ag8585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zona pellucida binding protein
Synonyms SP38, zona pellucida binding protein, ZPBP, ZPBP1
Calculated Molecular Weight 351 aa, 40 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC005223
Gene Symbol ZPBP
Gene ID (NCBI) 11055
RRIDAB_2878189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BS86
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZPBP (also known as ZPBP1), named for its binding to the oocyte zona pellucida, is a spermatid and spermatozoon protein that localizes to the acrosome. ZPBP may be implicated in gamete interaction during fertilization. It has been reported that absence of ZPBP1 prevents acrosome compaction and leads to acrosome fragmentation and sterility in male mice. (PMID: 17664285; 19204925)

实验方案

Product Specific Protocols
IP protocol for ZPBP antibody 15835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols