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验证数据展示

  • WB analysis using 19926-1-AP

    WB analysis using 19926-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19926-1-AP (ZSCAN5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 19926-1-AP

    IHC staining of human testis using 19926-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19926-1-AP (ZSCAN5A antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human testis using 19926-1-AP

    IHC staining of human testis using 19926-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19926-1-AP (ZSCAN5A antibody) at dilution of 1:200 (under 10x lens).

ZSCAN5A Polyclonal antibody

ZSCAN5A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19926-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Zinc finger protein 495, ZNF495, ZSCAN5, ZSCAN5A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19926-1-AP targets ZSCAN5A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ZSCAN5A fusion protein Ag13898 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and SCAN domain containing 5A
别名 Zinc finger protein 495, ZNF495, ZSCAN5, ZSCAN5A
计算分子量 496 aa, 56 kDa
GenBank蛋白编号BC002636
基因名称 ZSCAN5A
Gene ID (NCBI) 79149
RRIDAB_2878624
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUG6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger and SCAN domain-containing protein 5A (ZSCAN5A), also known as ZNF495 or ZSCAN5, belongs to the ZSCAN family of transcription factors. ZSCAN transcription factors contain two major domains, the zinc finger domain and the SCAN box. ZSCAN5A has two isoforms and is predicted to be located in the nucleus, it is also predicted to be involved in the regulation of transcription by RNA polymerase II and may be involved in angiogenesis, cell apoptosis, differentiation, migration and invasion, proliferation (PMID: 31218096).

实验方案

Product Specific Protocols
WB protocol for ZSCAN5A antibody 19926-1-APDownload protocol
IHC protocol for ZSCAN5A antibody 19926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols