验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, mouse testis tissue, K-562 cells, rat liver tissue, rat testis tissue |
| Positive IP detected in | Jurkat cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24561-1-AP targets ZW10 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, monkey |
| 免疫原 | ZW10 fusion protein Ag19138 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ZW10, kinetochore associated, homolog (Drosophila) |
| 别名 | HZW10 |
| 计算分子量 | 779 aa, 89 kDa |
| 观测分子量 | 88-90 kDa |
| GenBank蛋白编号 | BC128264 |
| 基因名称 | ZW10 |
| Gene ID (NCBI) | 9183 |
| RRID | AB_2815001 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43264 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZW10 is the human homolog of the Drosophila melanogaster Zw10 protein. ZW10 is localized to kinetochores and kinetochore-associated microtubules and is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis(PMID: 17102640). ZW10 proteins in Drosophila and HeLa cells display a similar and intriguing cell cycle-dependent intracellular distribution. ZW10 protein first becomes localized to the kinetochore at prometaphase, but then appears to move onto the kinetochore microtubules of the spindle at metaphase, and then back to the kinetochore at anaphase(PMID: 9700164).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZW10 antibody 24561-1-AP | Download protocol |
| IF protocol for ZW10 antibody 24561-1-AP | Download protocol |
| IP protocol for ZW10 antibody 24561-1-AP | Download protocol |
| FC protocol for ZW10 antibody 24561-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia | ||
Nat Commun Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2. | ||
J Cell Biol Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions. | ||
Cell Rep The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation.
| ||
iScience TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment |
